1. An electrophoretic mobility shift assay (EMSA) was performed using a radiolabeled DNA fragment from the sequence upstream of gene X. This DNA probe was incubated with (+) or without (-) nuclear extract isolated from tissues A (bone); B (lung); C (brain); and D (skin). The DNA;protein complexes were then fractionated on nondenaturing gels. The gels were exposed to autoradiographic film; the results are presented in the figure.
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