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EDS 1022 Strawberry DNA Isolation Lab (worth 50 points) Objective Students will isolate DNA from a strawberry by performing an at-home lab...


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I need some help with this report.  Strawberry DNA Isolation Lab.  I have pulled the DNA from the Strawberry already I just need help creating the Lab Report.



EDS 1022

 

Strawberry DNA Isolation Lab

 

(worth 50 points)

 

Objective

 

Students will isolate DNA from a strawberry by performing an at-home lab experiment, photograph their results, and

 

formalize their experience by writing a lab report.

 

Background Information

 

Before beginning the experiment, review chapters 23 and 24 of the course textbook, and watch the video provided on

 

the course website showing the extraction of strawberry DNA (an example of the experiment that you will be

 

completing here).

 

Assignment Instructions

 

1. Follow the instructions below to extract DNA from a strawberry. Read through the instructions completely before

 

starting the experiment.

 

Materials

 

two seal-able quart-size plastic bags (Ziploc™ type)

 

one strawberry with the leaves detached

 

(You can also use frozen strawberries, bananas, or split peas if fresh strawberries are out of season.)

 

one coffee filter

 

dish soap

 

salt

 

tap water

 

2 oz (1/4 cup) freezer-cold rubbing alcohol

 

one coffee stirrer or toothpick Procedure

 

a. Place the strawberry into one of the seal-able plastic bags. Once in the bag and sealed, squeeze and squish the

 

strawberry through the bag for two minutes. Be careful not to rupture the bag.

 

b. Put a small squirt of dish soap and small amount of salt (amounts do not need to be exact) into 4 oz (1/2 cup) of

 

water. Mix the soap and salt in the water without forming a lot of bubbles.

 

c. Add the soapy, salty solution into the seal-able bag with the strawberry. Seal the bag and squeeze/squish for

 

two more minutes.

 

d. Place the coffee filter into the second seal-able bag. Pour the strawberry mixture through the filter into the

 

second bag (you may need a lab assistant for this part). Do your best to not allow it to leak. Not all of the

 

contents from the first bag need to be filtered into the second bag.

 

e. Layer the freezer-cold rubbing alcohol over the filtered mixture in the second bag, by allowing it to drip down

 

the inside of the bag. Write down what you observe to happen once the alcohol is added. f. Spool out the white, stringy precipitant that forms in the bag with the toothpick or coffee stirrer – this is the

 

strawberry DNA.

 

g. Take a photo of the extracted DNA, which you will submit along with your lab report for this assignment.

 

2. Write a lab report which contains the following sections, presented in this order. Label each section of the report

 

clearly in your write-up.

 

Title Page – Come up with a title that specifically and accurately represents what the report is about. Center the

 

title, your name, and the date on the title page.

 

Abstract – write a brief and concise summary (100-150 word paragraph) of the experiment’s objectives, methods,

 

results, and conclusions. NOTE: this section comes first, but should be completed LAST, since it is a summary your

 

work.

 

Introduction – Provide some background (about a 200 word paragraph) on DNA and its applications in the modern

 

world. Discuss what DNA is, its role in living organisms, and some applications of DNA in criminology, medicine, etc.,

 

as discussed in chapter 24.

 

Materials and Methods – This section should include all information necessary to repeat the experiment (materials

 

needed (including amounts) and steps of the experiment procedure), written in narrative in form. In describing the

 

methods (or procedure), include the following:

 

For each of the following steps in the experiment procedure, Explain, in 2-3 sentences, the specific reason that

 

the step was necessary for the extraction of the strawberry DNA. Cite any sources used in researching the

 

reasons.

 

- Initial mashing/squishing of the strawberries

 

Adding the soap and salt solution and continued mashing

 

Straining the strawberry mixture through the coffee filter

 

Adding the freezer-cold rubbing alcohol Results – A discussion (150-200 word paragraph) of the observations made during the experiment, in narrative form.

 

In this section, explain what happened in the final step when you added the rubbing alcohol to your strawberry

 

extract. The white, stringy material that precipitated out was the strawberry DNA. Relate what you know about the

 

chemical structure of DNA to what you observed.

 

Discussion – Your interpretation (150-200 word paragraph) of the experiment and results. Describe your experience

 

while completing the steps of the experiment. What parts were difficult to complete? Did you have to repeat the

 

experiment for any reason? Did you expect, or were you surprised, by the results? 3. Submit your completed lab report AND photo of the extracted DNA to the designated digital drop box on the course

 

website. The report is worth 40 points and the photo is worth 10 points.

 


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